The following guidance is based on the best available evidence. The full guideline gives details of the methods and the evidence used to develop the guidance.
Aseptic technique An aseptic technique ensures that only uncontaminated equipment and fluids come into contact with susceptible body sites. It should be used during any clinical procedure that bypasses the body's natural defences. Using the principles of asepsis minimises the spread of organisms from one person to another.
Direct patient care 'Hands on' or face-to-face contact with patients. Any physical aspect of the healthcare of a patient, including treatments, self-care and administration of medication.
Hand decontamination The use of handrub or handwashing to reduce the number of bacteria on the hands. In this guideline this term is interchangeable with 'hand hygiene'.
Handrub A preparation applied to the hands to reduce the number of viable microorganisms. This guideline refers to handrubs compliant with British standards (BS EN1500; standard for efficacy of hygienic handrubs using a reference of 60% isopropyl alcohol).
Healthcare worker Any person employed by the health service, social services, a local authority or an agency to provide care for a sick, disabled or elderly person.
Healthcare waste In this guideline, healthcare waste refers to any waste produced by, and as a consequence of, healthcare activities.
Personal protective equipment Equipment that is intended to be worn or held by a person to protect them from risks to their health and safety while at work. Examples include gloves, aprons, and eye and face protection.
220.127.116.11 Everyone involved in providing care should be:
materials for hand decontamination
personal protective equipment. [new 2012]
18.104.22.168 Educate patients and carers about:
the benefits of effective hand decontamination
the correct techniques and timing of hand decontamination
when it is appropriate to use liquid soap and water or handrub
the availability of hand decontamination facilities
their role in maintaining standards of healthcare workers' hand decontamination. [new 2012]
22.214.171.124 Hands must be decontaminated in all of the following circumstances:
immediately before every episode of direct patient contact or care, including aseptic procedures
immediately after every episode of direct patient contact or care
immediately after any exposure to body fluids
immediately after any other activity or contact with a patient's surroundings that could potentially result in hands becoming contaminated
immediately after removal of gloves. [new 2012]
126.96.36.199 Decontaminate hands preferably with a handrub (conforming to current British standards), except in the following circumstances, when liquid soap and water must be used:
when hands are visibly soiled or potentially contaminated with body fluids or
in clinical situations where there is potential for the spread of alcohol-resistant organisms (such as Clostridium difficile or other organisms that cause diarrhoeal illness). [new 2012]
188.8.131.52 Healthcare workers should ensure that their hands can be decontaminated throughout the duration of clinical work by:
184.108.40.206 An effective handwashing technique involves three stages: preparation, washing and rinsing, and drying. Preparation requires wetting hands under tepid running water before applying liquid soap or an antimicrobial preparation. The handwash solution must come into contact with all of the surfaces of the hand. The hands must be rubbed together vigorously for a minimum of 10–15 seconds, paying particular attention to the tips of the fingers, the thumbs and the areas between the fingers. Hands should be rinsed thoroughly before drying with good quality paper towels. 
220.127.116.11 When decontaminating hands using an alcohol handrub, hands should be free from dirt and organic material. The handrub solution must come into contact with all surfaces of the hand. The hands must be rubbed together vigorously, paying particular attention to the tips of the fingers, the thumbs and the areas between the fingers, until the solution has evaporated and the hands are dry. 
18.104.22.168 An emollient hand cream should be applied regularly to protect skin from the drying effects of regular hand decontamination. If a particular soap, antimicrobial hand wash or alcohol product causes skin irritation an occupational health team should be consulted. 
22.214.171.124 Selection of protective equipment must be based on an assessment of the risk of transmission of microorganisms to the patient, and the risk of contamination of the healthcare worker's clothing and skin by patients' blood, body fluids, secretions or excretions. 
126.96.36.199 Gloves used for direct patient care:
188.8.131.52 Gloves must be worn for invasive procedures, contact with sterile sites and non-intact skin or mucous membranes, and all activities that have been assessed as carrying a risk of exposure to blood, body fluids, secretions or excretions, or to sharp or contaminated instruments. 
184.108.40.206 Gloves must be worn as single-use items. They must be put on immediately before an episode of patient contact or treatment and removed as soon as the activity is completed. Gloves must be changed between caring for different patients, and between different care or treatment activities for the same patient. 
220.127.116.11 Ensure that gloves used for direct patient care that have been exposed to body fluids are disposed of correctly, in accordance with current national legislation or local policies (see section 1.1.5). [new 2012]
18.104.22.168 Alternatives to natural rubber latex gloves must be available for patients, carers and healthcare workers who have a documented sensitivity to natural rubber latex. 
22.214.171.124 Do not use polythene gloves for clinical interventions. [new 2012]
126.96.36.199 When delivering direct patient care:
wear a disposable plastic apron if there is a risk that clothing may be exposed to blood, body fluids, secretions or excretions or
wear a long-sleeved fluid-repellent gown if there is a risk of extensive splashing of blood, body fluids, secretions or excretions onto skin or clothing. 
188.8.131.52 When using disposable plastic aprons or gowns:
use them as single-use items, for one procedure or one episode of direct patient care and
ensure they are disposed of correctly (see section 1.1.5). 
184.108.40.206 Face masks and eye protection must be worn where there is a risk of blood, body fluids, secretions or excretions splashing into the face and eyes. 
220.127.116.11 Respiratory protective equipment, for example a particulate filter mask, must be used when clinically indicated. 
18.104.22.168 Sharps should not be passed directly from hand to hand, and handling should be kept to a minimum. [2003, amended 2012]
22.214.171.124 Used standard needles:
must not be bent or broken before disposal
must not be recapped.
In dentistry, if recapping or disassembly is unavoidable, a risk assessment must be undertaken and appropriate safety devices should be used. [new 2012]
126.96.36.199 Used sharps must be discarded immediately by the person generating the sharps waste into a sharps container conforming to current standards. [new 2012]
188.8.131.52 Sharps containers:
must be located in a safe position that avoids spillage, is at a height that allows the safe disposal of sharps, is away from public access areas and is out of the reach of children
must notbe used for any other purpose than the disposal of sharps
must notbe filled above the fill line
must be disposed of when the fill line is reached
should be temporarily closed when not in use
should be disposed of every 3 months even if not full, by the licensed route in accordance with local policy. [new 2012]
184.108.40.206 Use sharps safety devices if a risk assessment has indicated that they will provide safer systems of working for healthcare workers, carers and patients. [new 2012]
220.127.116.11 Train and assess all users in the correct use and disposal of sharps and sharps safety devices. [new 2012]
18.104.22.168 Healthcare waste must be segregated immediately by the person generating the waste into appropriate colour-coded storage or waste disposal bags or containers defined as being compliant with current national legislation and local policies. [new 2012]
22.214.171.124 Healthcare waste must be labelled, stored, transported and disposed of in accordance with current national legislation and local policies. [new 2012]
126.96.36.199 Educate patients and carers about the correct handling, storage and disposal of healthcare waste. [new 2012]
188.8.131.52 Patients and carers should be educated about and trained in techniques of hand decontamination, insertion of intermittent catheters where applicable, and catheter management before discharge from hospital. 
184.108.40.206 Community and primary healthcare workers must be trained in catheter insertion, including suprapubic catheter replacement and catheter maintenance. 
220.127.116.11 Follow-up training and ongoing support of patients and carers should be available for the duration of long-term catheterisation. 
18.104.22.168 Indwelling urinary catheters should be used only after alternative methods of management have been considered. 
22.214.171.124 The patient's clinical need for catheterisation should be reviewed regularly and the urinary catheter removed as soon as possible. 
126.96.36.199 Catheter insertion, changes and care should be documented. 
188.8.131.52 Following assessment, the best approach to catheterisation that takes account of clinical need, anticipated duration of catheterisation, patient preference and risk of infection should be selected. 
184.108.40.206 Intermittent catheterisation should be used in preference to an indwelling catheter if it is clinically appropriate and a practical option for the patient. 
220.127.116.11 Offer a choice of either single-use hydrophilic or gel reservoir catheters for intermittent self-catheterisation. [new 2012]
18.104.22.168 Select the type and gauge of an indwelling urinary catheter based on an assessment of the patient's individual characteristics, including:
any allergy or sensitivity to catheter materials
history of symptomatic urinary tract infection
patient preference and comfort
previous catheter history
reason for catheterisation. [new 2012]
22.214.171.124 In general, the catheter balloon should be inflated with 10 ml of sterile water in adults and 3–5 ml in children. 
126.96.36.199 In patients for whom it is appropriate, a catheter valve may be used as an alternative to a drainage bag. 
188.8.131.52 All catheterisations carried out by healthcare workers should be aseptic procedures. After training, healthcare workers should be assessed for their competence to carry out these types of procedures. 
184.108.40.206 Intermittent self-catheterisation is a clean procedure. A lubricant for single-patient use is required for non-lubricated catheters. 
220.127.116.11 For urethral catheterisation, the meatus should be cleaned before insertion of the catheter, in accordance with local guidelines/policy. 
18.104.22.168 An appropriate lubricant from a single-use container should be used during catheter insertion to minimise urethral trauma and infection. 
22.214.171.124 Indwelling catheters should be connected to a sterile closed urinary drainage system or catheter valve. 
126.96.36.199 Healthcare workers should ensure that the connection between the catheter and the urinary drainage system is not broken except for good clinical reasons (for example changing the bag in line with the manufacturer's recommendations). 
188.8.131.52 Healthcare workers must decontaminate their hands and wear a new pair of clean, non-sterile gloves before manipulating a patient's catheter, and must decontaminate their hands after removing gloves. 
184.108.40.206 Patients managing their own catheters, and their carers, must be educated about the need for hand decontamination before and after manipulation of the catheter, in accordance with the recommendations in the standard principles section (section 1.1). [2003, amended 2012]
220.127.116.11 Urine samples must be obtained from a sampling port using an aseptic technique. 
18.104.22.168 Urinary drainage bags should be positioned below the level of the bladder, and should not be in contact with the floor. 
22.214.171.124 A link system should be used to facilitate overnight drainage, to keep the original system intact. 
126.96.36.199 The urinary drainage bag should be emptied frequently enough to maintain urine flow and prevent reflux, and should be changed when clinically indicated. 
188.8.131.52 The meatus should be washed daily with soap and water. 
184.108.40.206 To minimise the risk of blockages, encrustations and catheter-associated infections for patients with a long-term indwelling urinary catheter:
develop a patient-specific care regimen
consider approaches such as reviewing the frequency of planned catheter changes and increasing fluid intake
document catheter blockages. [new 2012]
220.127.116.11 Bladder instillations or washouts must not be used to prevent catheter-associated infections. 
18.104.22.168 Catheters should be changed only when clinically necessary or according to the manufacturer's current recommendations. 
22.214.171.124 When changing catheters in patients with a long-term indwelling urinary catheter:
126.96.36.199 Patients and carers should be educated about and trained in the techniques of hand decontamination, enteral feeding and the management of the administration system before being discharged from hospital. 
188.8.131.52 Healthcare workers should be trained in enteral feeding and management of the administration system. 
184.108.40.206 Follow-up training and ongoing support of patients and carers should be available for the duration of home enteral tube feeding. 
220.127.116.11 Wherever possible pre-packaged, ready-to-use feeds should be used in preference to feeds requiring decanting, reconstitution or dilution. 
18.104.22.168 The system selected should require minimal handling to assemble, and be compatible with the patient's enteral feeding tube. 
22.214.171.124 Effective hand decontamination must be carried out before starting feed preparation. 
126.96.36.199 When decanting, reconstituting or diluting feeds, a clean working area should be prepared and equipment dedicated for enteral feed use only should be used. 
188.8.131.52 Feeds should be mixed using cooled boiled water or freshly opened sterile water and a no-touch technique. 
184.108.40.206 Feeds should be stored according to the manufacturer's instructions and, where applicable, food hygiene legislation. 
220.127.116.11 Where ready-to-use feeds are not available, feeds may be prepared in advance, stored in a refrigerator, and used within 24 hours. 
18.104.22.168 Use minimal handling and an aseptic technique to connect the administration system to the enteral feeding tube. [new 2012]
22.214.171.124 Ready-to-use feeds may be given for a whole administration session, up to a maximum of 24 hours. Reconstituted feeds should be administered over a maximum 4-hour period. 
126.96.36.199 Administration sets and feed containers are for single use and must be discarded after each feeding session. 
188.8.131.52 The stoma should be washed daily with water and dried thoroughly. 
184.108.40.206 To prevent blockages, flush the enteral feeding tube before and after feeding or administering medications using single-use syringes or single-patient-use (reusable) syringes according to the manufacturer's instructions. Use:
freshly drawn tap water for patients who are not immunosuppressed
either cooled freshly boiled water or sterile water from a freshly opened container for patients who are immunosuppressed. [new 2012]
220.127.116.11 Before discharge from hospital, patients and their carers should be taught any techniques they may need to use to prevent infection and safely manage a vascular access device. [2003, amended 2012]
18.104.22.168 Healthcare workers caring for a patient with a vascular access device should be trained, and assessed as competent, in using and consistently adhering to the infection prevention practices described in this guideline. [2003, amended 2012]
22.214.171.124 Follow-up training and support should be available to patients with a vascular access device and their carers. [2003, amended 2012]
126.96.36.199 Hands must be decontaminated (see section 1.1.2) before accessing or dressing a vascular access device. [new 2012]
188.8.131.52 An aseptic technique must be used for vascular access device catheter site care and when accessing the system. [new 2012]
184.108.40.206 Decontaminate the skin at the insertion site with chlorhexidine gluconate in 70% alcohol before inserting a peripheral vascular access device or a peripherally inserted central catheter. [new 2012]
220.127.116.11 Use a sterile transparent semipermeable membrane dressing to cover the vascular access device insertion site. [new 2012]
18.104.22.168 Consider a sterile gauze dressing covered with a sterile transparent semipermeable membrane dressing only if the patient has profuse perspiration, or if the vascular access device insertion site is bleeding or oozing. If a gauze dressing is used:
change it every 24 hours, or sooner if it is soiled and
replace it with a sterile transparent semipermeable membrane dressing as soon as possible. [new 2012]
22.214.171.124 Change the transparent semipermeable membrane dressing covering a central venous access device insertion site every 7 days, or sooner if the dressing is no longer intact or moisture collects under it. 
126.96.36.199 Leave the transparent semipermeable membrane dressing applied to a peripheral cannula insertion site in situ for the life of the cannula, provided that the integrity of the dressing is retained. [new 2012]
188.8.131.52 Dressings used on tunnelled or implanted central venous catheter sites should be replaced every 7 days until the insertion site has healed, unless there is an indication to change them sooner. 
184.108.40.206 Healthcare workers should ensure that catheter-site care is compatible with catheter materials (tubing, hubs, injection ports, luer connectors and extensions) and carefully check compatibility with the manufacturer's recommendations. 
220.127.116.11 Decontaminate the central venous catheter insertion site and surrounding skin during dressing changes using chlorhexidine gluconate in 70% alcohol, and allow to air dry. Consider using an aqueous solution of chlorhexidine gluconate if the manufacturer's recommendations prohibit the use of alcohol with their catheter. 
18.104.22.168 Individual sachets of antiseptic solution or individual packages of antiseptic-impregnated swabs or wipes should be used to disinfect the dressing site. 
22.214.171.124 Decontaminate the injection port or vascular access device catheter hub before and after accessing the system using chlorhexidine gluconate in 70% alcohol. Consider using an aqueous solution of chlorhexidine gluconate if the manufacturer's recommendations prohibit the use of alcohol with their catheter. [new 2012]
126.96.36.199 In-line filters should not be used routinely for infection prevention. 
188.8.131.52 Antibiotic lock solutions should not be used routinely to prevent catheter-related bloodstream infections (CRBSI). 
184.108.40.206 Systemic antimicrobial prophylaxis should not be used routinely to prevent catheter colonisation or CRBSI, either before insertion or during the use of a central venous catheter. 
220.127.116.11 Preferably, a single lumen catheter should be used to administer parenteral nutrition. If a multilumen catheter is used, one port must be exclusively dedicated for total parenteral nutrition, and all lumens must be handled with the same meticulous attention to aseptic technique. 
18.104.22.168 Preferably, a sterile 0.9 percent sodium chloride injection should be used to flush and lock catheter lumens. 
22.214.171.124 When recommended by the manufacturer, implanted ports or opened-ended catheter lumens should be flushed and locked with heparin sodium flush solutions. 
126.96.36.199 Systemic anticoagulants should not be used routinely to prevent CRBSI. 
188.8.131.52 If needleless devices are used, the manufacturer's recommendations for changing the needleless components should be followed. 
184.108.40.206 When needleless devices are used, healthcare workers should ensure that all components of the system are compatible and secured, to minimise leaks and breaks in the system. 
220.127.116.11 When needleless devices are used, the risk of contamination should be minimised by decontaminating the access port with either alcohol or an alcoholic solution of chlorhexidine gluconate before and after using it to access the system. 
18.104.22.168 In general, administration sets in continuous use need not be replaced more frequently than at 72-hour intervals unless they become disconnected or a catheter-related infection is suspected or documented. 
22.214.171.124 Administration sets for blood and blood components should be changed every 12 hours, or according to the manufacturer's recommendations. 
126.96.36.199 Administration sets used for total parenteral nutrition infusions should generally be changed every 24 hours. If the solution contains only glucose and amino acids, administration sets in continuous use do not need to be replaced more frequently than every 72 hours. 
188.8.131.52 Avoid the use of multidose vials, in order to prevent the contamination of infusates. [new 2012]
 In accordance with current health and safety legislation (at the time of publication of the guideline [March 2012]): Health and Safety at Work Act 1974, Management of Health and Safety at Work Regulations 1999, Health and Safety Regulations 2002, Control of Substances Hazardous to Health Regulations 2002, Personal Protective Equipment Regulations 2002 and Health and Social Care Act 2008.
 At the time of publication of the guideline (March 2012): BS EN 1500:1997.
 For the purposes of this guideline, the GDG considered bare below the elbow to mean: not wearing false nails or nail polish; not wearing a wrist-watch or stoned rings; wearing short-sleeved garments or being able to roll or push up sleeves.
 At the time of publication of the guideline (March 2012): BS EN 455 Parts 1–4 Medical gloves for single use.
 The updated recommendation contains 'should' rather than 'must' (which is in the 2003 guideline) because the GDG considered that this is not covered by legislation (in accordance with the NICE guidelines manual, 2009).
 It is acceptable to bend needles when they are part of an approved sharps safety device.
 At the time of publication of the guideline (March 2012): UN3291 and BS 7320.
 The text 'Patients managing their own catheters, and their carers, must be educated about the need for hand decontamination…' has replaced 'Carers and patients managing their own catheters must wash their hands…' in the 2003 guideline.
 At the time of publication of the guideline (March 2012), no antibiotics have a UK marketing authorisation for this indication. Informed consent should be obtained and documented.
 The GDG defined trauma as frank haematuria after catheterisation or two or more attempts of catheterisation.
 The updated recommendation contains 'vascular access device' rather than 'central venous catheter'. This change has been made because peripherally inserted catheters were included in the scope of the guideline update.
 The GDG considered that Aseptic Non Touch Technique (ANTT™) is an example of an aseptic technique for vascular access device maintenance, which is widely used in acute and community settings and represents a possible framework for establishing standardised guidance on aseptic technique.