Tools and resources

Adoption support resource – insights from the NHS

8 The technologies

8 The technologies

Several faecal calprotectin tests are available to the NHS in England, including fully quantitative laboratory‑based technologies (many of which use an enzyme‑linked immunosorbent assay [ELISA] platform), fully quantitative rapid tests and semi‑quantitative point‑of‑care tests (POCTs). Table 9 details the 12 tests that are included in the guidance. The reference standard was histology after endoscopy.

Table 9 Faecal calprotectin technologies included in the assessment for guidance [2]

Manufacturer

Test

Platform

Buhlmann

EK‑CAL calprotectin ELISA test

ELISA – quantitative

Range (depending on dilution):

10–600 mcg/g

30–1800 mcg/g.

Buhlmann

LF‑CAL25 Quantum Blue calprotectin test

Rapid test – immunoassay designed for the quantitative determination of faecal calprotectin in combination with the Buhlmann Quantum Blue reader.

Range: 30–300 mcg/g.

Buhlmann

LF‑CHR 25 Quantum Blue calprotectin test

Rapid test – immunoassay designed for the quantitative determination of faecal calprotectin in combination with the Buhlmann Quantum Blue reader.

Range: 100–1800 mcg/g.

Calpro

CALPRO CALPROTECTIN ELISA TEST (ALP) – formerly known as the Phical test

CAL0100

ELISA – quantitative

Range: up to 1250 mg/kg.

Calpro

CALPROLAB CALPROTECTIN ELISA (ALP) – formerly known as the Phical test

CALP0170

ELISA – quantitative

Range: up to 2500 mg/kg.

Eurospital

Calprest

ELISA – quantitative.

Eurospital

CalFast

Rapid test – quantitative determination of faecal calprotectin in combination with a dedicated reader.

Immundiagnostik

ELISA (K 6927)

ELISA – quantitative.

Phadia AB, part of Thermo Fisher Scientific

EliA Calprotectin

EliA – quantitative

Quantitative fluorescence enzyme immunoassay (FEIA) test.

Range: 15–3000 mg/kg.

Preventis (sister company to Immundiagnostik)

KST11005 PreventID CalDetect Calprotectin Rapid test (version 1 – Caldetect)

POCT – immunochromatographic rapid test.

A semi‑quantitative test with 3 lines corresponding to:

Calprotectin 'negative'

Calprotectin≤15 mcg/g

Calprotectin 16–60 mcg/g

Calprotectin>60 mcg/g stool.

Preventis (sister company to Immundiagnostik)

KST11004

PreventID CalScreen

Calprotectin Rapid test (version 3 – CalScreen)

POCT – immunochromatographic rapid test.

A yes/no test with only 1 test‑line corresponding to the cut‑off value of 50 mcg/g stool (no inflammation=<50 mcg/g and inflammation present=≥50 mcg/g).

Abbreviations: ELISA, enzyme‑linked immunosorbent assay; mcg, micrograms; POCT, point‑of‑care test.

While assaying calprotectin, laboratories should be aware of the lack of international standardisation (as demonstrated by the between‑assay variability), of the high biological variation and of the criticality of the extraction.

For further details and up‑to‑date costs please contact the relevant company.

Contact details


[2] The names of the tests used by sites were correct at the time of publication. However, since DG11 published, several of the faecal calprotectin tests mentioned in the guidance document have been renamed. Details of the changes can be found in table 1, appendix 1 of the guidance review decision.


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